This study was undertaken to produce and quantify anthocyanins in Karadanfi red sorghum in vitro. Mature embryo and immature embryo explants were used for callus induction using Murashige and Skoog basal medium supplemented with varying concentrations of 2, 4-D (0 mg/L - 4.0 mg/L) alone or in combination with 0.5 mg/L KN. Immature embryo was used for regeneration using MS media supplemented with BA (0 mg/L - 3 mg/L) alone or in combination with 2, 4-D (0.1 mg/L). The calli obtained were sub cultured on the media fortified with BA (0 mg/L - 2 mg/L) and NAA (0.2 mg/L) for anthocyanins production. Results indicated highly significant difference for percentage callus formation, callus fresh weight, total and monomeric anthocyanins. Significant differences were also observed for percentage shoot initiation. Medium fortified with 2 mg/L 2, 4-D alone or in combination with 0.5 mg/L kinetin was found to be optimum for callus induction. It was observed from this study that more 3-deoxyanthocyanindin can be obtained from in vitro grown sorghum at callus stage. The highest level of anthocyanin was produced in this study using MS media fortified with 1.0 mg/L BA. It is therefore concluded that in vitro system is suitable for the production of anthocyanin pigment in Karandafi red sorghum.