This study was carried out to determine the "effects of Simulation game on Attitude and Performance in Biology among Secondary School Students, Zaria, Kaduna State Nigeria. The target population was all SS1 Biology students in Zaria Educational zone. Two Schools were sampledusing the stratified random sampling techniques. Intact Classes were used, where 153 subjects of 94 males and 59 females were sampled from the total population of 2,005. Three research questions and three null hypotheses guided the conduct of the study. The hypotheses were tested at ?≤0.05 level of significance. A pretest and posttest quasi experimental control design was used. The experimental group was taught microorganism using the simulation game strategy, while the control group was exposed to lecture method. Two validated instrumentsMicroorganisms Performance Test (MOPT) and Biology Attitude Questionnaire (BAQ)were used for both Pretest and Post-test.The reliability of the Instruments was calculated using Split-half method of Gutman. Based on the analysis of the Scores obtained from the Pilot testing of the Microorganisms Performance Test (MOPT), the reliability coefficient for the test was 0.82, while that of Biology Attitude Questionnaire (BAQ)was0.75. The two Instruments were used to collect data, which were analyzed using independent t-test, Two-Way Analysis of Variance and Kruskai Wallis. The findings revealed that (1). There was a significant difference in the mean scores of the experimental group when taught microorganisms using simulation game strategy compared to their counterparts in the control group when taught using lecture method. (ii) There was a significant difference between the attitude change of the students before and after exposure to simulation game strategy. (iii) There was no significant difference interms of gender academic performance of male and female students when taught using simulation game strategy and those taught using lecture method.Among the recommendations given, was that simulation game strategy should be encouraged for the teaching and learning of Biology at the secondary school level. Professional associations and educational researches, should organize seminars etc, to sensitize the need for adaptation of the simulation game strategy at the secondary school level.